Temporary

Part:BBa_J70367:Design

Designed by: Julie Norville   Group: Knight Lab   (2009-06-28)

PsrI Invariant Green {β,0,5,-5,PsrI;β,5,0,5,PsrI}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 883


Design Notes

Cuts shoudn't interfere 


left cut site   left                       left cut site right

    ctaga^gatacat                      tac gcaaaccg   cctct  ^

^ gatct ctatgta atg cgttt ggc^ggaga 


right cut site left                     right cut right side   

  ttgtt  ^  tgtcggt gaac             tac tagagtc     tacta ^

^aacaa    acagcca cttc              atg atctcag  ^ atgat



Source

J04430

References